Phosphatidylserine-containing liposomes promote maximal survival of retinal neurons after ischemic injury
This study was supported by the AHA Scientist Development Award 0735014B (DI); NIH grant EY017991 and Research to Prevent Blindness (RPB) Career Development Award (VS); NIH grant P30 EY014801 and unrestricted RPB grant to the University of Miami Department of Ophthalmology.
Galina Dvoriantchikova1, Christian Agudelo2, Eleut Hernandez1, Valery I Shestopalov1,3 and Dmitry Ivanov1,4
1Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, USA
2Department of Physiology and Biophysics, University of Miami Miller School of Medicine, Miami, Florida, USA
3Departments of Cell Biology and Anatomy, University of Miami Miller School of Medicine, Miami, Florida, USA
4Vavilov Institute of General Genetics RAS, Moscow, Russian Federation
Correspondence: Dr D Ivanov, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, 1638 NW 10th Ave, Miami, FL 33136, USA. E-mail: firstname.lastname@example.org
; Dr V Shestopalov, E-mail: email@example.com
Received 27 April 2009; Revised 20 June 2009; Accepted 22 June 2009; Published online 15 July 2009.
Top of pageAbstract
We investigated the systemic effect of liposomes bearing apoptotic signals on the level of inflammation and neuronal death induced by ischemia–reperfusion (IR). Using a model of retinal ischemia, we showed that treatment with phosphatidylserine (PS) and phosphatidylcholine (PC) liposomes significantly reduced the expression of proinflammatory genes, including that of Il1b, Il6, Ccl2, Ccl5, Cxcl10, and Icam1, 24 h after reperfusion. Phosphatidylserine liposome treatment was the most efficient and correlated with significantly reduced neuronal death in the retina 7 days after reperfusion. The results of our study indicate that therapeutic strategy based on mimicking a systemic increase in apoptotic signaling can significantly reduce central nervous system damage induced by IR and improve neurologic outcome.
Keywords: apoptosis, inflammation, ischemia, liposomes, phosphatidylserine, retinal pathology
http://www.nature.com/jcbfm/journal/v29 ... 0995a.html