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Bacterium Clostridium perfringens may be MS trigger
Posted: Fri Oct 18, 2013 12:29 am
by slghmevp
We have isolated Clostridium perfringens type B, an epsilon toxin-secreting bacillus, from a young woman at clinical presentation of Multiple Sclerosis (MS) with actively enhancing lesions on brain MRI. This finding represents the first time that C. perfringens type B has been detected in a human. Epsilon toxin’s tropism for the blood-brain barrier (BBB) and binding to oligodendrocytes/myelin makes it a provocative candidate for nascent lesion formation in MS. We examined a well-characterized population of MS patients and healthy controls for carriage of C. perfringens toxinotypes in the gastrointestinal tract. The human commensal Clostridium perfringens type A was present in approximately 50% of healthy human controls compared to only 23% in MS patients. We examined sera and CSF obtained from two tissue banks and found that immunoreactivity to ETX is 10 times more prevalent in people with MS than in healthy controls, indicating prior exposure to ETX in the MS population. C. perfringens epsilon toxin fits mechanistically with nascent MS lesion formation since these lesions are characterized by BBB permeability and oligodendrocyte cell death in the absence of an adaptive immune infiltrate.
http://www.plosone.org/article/info%3Ad ... ne.0076359
Re: Bacterium Clostridium perfringens may be MS trigger
Posted: Fri Oct 18, 2013 12:55 am
by cervocuit
Have they found the occult GI phatogen driving MS symptomes that Thomas Borody was talking about ?
From the abstract of MSRC
http://www.ms-uk.org/MSnews
...
"But one of my favorite approaches is development of a probiotic cocktail that delivers bacteria that compete with, and destroy, C. perfringens types B and D," Vartanian says. "It would be such a beautiful and natural way to treat the gastrointestinal system and solve the problem. We are also starting to work on this approach."
The ultimate probiotic cocktail already exist inside healthy persons and can be easily transplanted.
http://www.thisisms.com/forum/general-d ... 20297.html
Re: Bacterium Clostridium perfringens may be MS trigger
Posted: Fri Oct 18, 2013 2:09 am
by Leonard
here is another article
http://weill.cornell.edu/news/releases/ ... 6_13.shtml
fascinating...
noted the 33 kD protoxin; perhaps this is the mysterious 36,000 Dalton toxic protein in Gay's paper...
Re: Bacterium Clostridium perfringens may be MS trigger
Posted: Sat Sep 20, 2014 3:25 am
by cervocuit
This research has been presented at ECTRIMS 2014.
http://www.opus3.com/MSBoston_PressKit/ ... ournal.pdf
p369
Also, in my quest of convincing myself to completely stop coffee, I found this:
http://www.ncbi.nlm.nih.gov/pubmed/6271685
Stimulation of Clostridium perfringens enterotoxin formation by caffeine and theobromine.
Abstract: In the presence of 100 micrograms of caffeine per ml or 200 micrograms of theobromine per ml, sporulation of Clostridium perfringens NCTC 8679 rose from less than 1 to 80 or 85%. Enterotoxin concentration increased from undetectable levels to 450 micrograms/mg of cell extract protein….
Re: Bacterium Clostridium perfringens may be MS trigger
Posted: Sun Sep 04, 2016 10:04 pm
by cervocuit
Gut microbiome analysis in neuromyelitis optica reveals overabundance of Clostridium perfringens.
http://www.ncbi.nlm.nih.gov/pubmed/27398819
Re: Bacterium Clostridium perfringens may be MS trigger
Posted: Mon Sep 05, 2016 1:28 am
by NHE
Re: Bacterium Clostridium perfringens may be MS trigger
Posted: Sun Sep 11, 2016 8:35 pm
by mrtmeo
Clostridium perfringens is easy killed off with iodine.
https://www.akademie-zwm.ch/uploads/med ... /18768.pdf
Re: Bacterium Clostridium perfringens may be MS trigger
Posted: Tue Apr 24, 2018 12:51 am
by cervocuit
Re: Bacterium Clostridium perfringens may be MS trigger
Posted: Tue Apr 24, 2018 4:04 am
by NHE
This bacterium is considerably more common than the above article suggests.
https://www.foodsafety.gov/poisoning/ca ... index.html
Clostridium perfringens (C. perfringens) is one of the most common causes of food poisoning in the United States. According to some estimates, this type of bacteria causes nearly a million illnesses each year.
Re: Bacterium Clostridium perfringens may be MS trigger
Posted: Tue Apr 24, 2018 4:57 am
by cervocuit
The bacteria is common but the problem is supposed to be the toxin created by this bacteria.
They found 43% of MS patients were carrying antibodies to epsilon toxin VS 16% for controls
Re: Bacterium Clostridium perfringens may be MS trigger
Posted: Thu Mar 07, 2019 2:46 am
by cervocuit
New study
Presented at ACTRIMS 2019
https://actrims.confex.com/actrims/2019 ... Paper/3996
- 21% of MS patients have presence of ETX producing bacteria, 0% in healthy controls.
- MS patients have more ETX in their blood.
- ETX administration in mice resulted in disease activity similar to MS (BBB permeability and focal demyelinisation).
“for our model of ETX-induced MS, we propose that ETX-producing bacteria colonize the human gastrointestinal tract, resulting in periodical blooms of exponential growth, leading to secretion of the toxin,"
https://multiplesclerosisnewstoday.com/ ... e-rodents/
Re: Bacterium Clostridium perfringens may be MS trigger
Posted: Tue Mar 12, 2019 7:12 am
by frodo
It seems that the original paper that opens this thread refers only to pattern III MS (balo-like lesions). They call it "nascent lesion formation" because back in 2013 some authors though that it was a pattern that could turn into anyone of the others. It does not apply anymore.
Quote from the paper: "... C. perfringens epsilon toxin fits mechanistically with
nascent MS lesion formation since these lesions are characterized by BBB permeability and
oligodendrocyte cell death in the absence of an adaptive immune infiltrate".
Anyway the possibility of this bacteria to be the culprit in that 23% of cases (which is close to the pattern III reported prevalence) is still possible.
Re: Bacterium Clostridium perfringens may be MS trigger
Posted: Wed Aug 07, 2019 11:43 am
by Petr75
2019 Jun 14
Leicester Institute of Structural and Chemical Biology, Department of Molecular and Cell Biology, University of Leicester, UK
The pore structure of Clostridium perfringens epsilon toxin.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572795/
Abstract
Epsilon toxin (Etx), a potent pore forming toxin (PFT) produced by Clostridium perfringens, is responsible for the pathogenesis of enterotoxaemia of ruminants and has been suggested to play a role in multiple sclerosis in humans. Etx is a member of the aerolysin family of β-PFTs (aβ-PFTs). While the Etx soluble monomer structure was solved in 2004, Etx pore structure has remained elusive due to the difficulty of isolating the pore complex. Here we show the cryo-electron microscopy structure of Etx pore assembled on the membrane of susceptible cells. The pore structure explains important mutant phenotypes and suggests that the double β-barrel, a common feature of the aβ-PFTs, may be an important structural element in driving efficient pore formation. These insights provide the framework for the development of novel therapeutics to prevent human and animal infections, and are relevant for nano-biotechnology applications.
Re: Bacterium Clostridium perfringens may be MS trigger
Posted: Thu Aug 08, 2019 4:35 am
by NHE
From the references section...
Isolation of Clostridium perfringens type B in an individual at first clinical presentation of multiple sclerosis provides clues for environmental triggers of the disease. PLoS ONE. 2013;8:e76359.
The myelin and lymphocyte protein MAL is required for binding and activity of Clostridium perfringens epsilon-Toxin.
PLoS Pathog. 2015;11:e1004896.
Evidence of Clostridium perfringens epsilon toxin associated with multiple sclerosis.
Mult. Scler. 2018;25:653–660.
Re: Bacterium Clostridium perfringens may be MS trigger
Posted: Sun Aug 25, 2019 12:07 pm
by dwsn
NHE wrote: ↑Tue Apr 24, 2018 4:04 am
This bacterium is considerably more common than the above article suggests.
https://www.foodsafety.gov/poisoning/ca ... index.html
Clostridium perfringens (C. perfringens) is one of the most common causes of food poisoning in the United States. According to some estimates, this type of bacteria causes nearly a million illnesses each year.
There are different types of C. perfringens bacteria based on the production of different toxins. This bacteria can produce close to 20 different toxins. The toxin identified as a common cause of food poisoning is called the "enterotoxin." The toxin that has been implicated in multiple sclerosis is called "epsilon toxin." C. perfringens type B and type D produce the epsilon toxin, and these epsilon toxin producing types of C. perfringens are not at all common in human beings; are not related to human food poisoning, but have recently been found in MS patients. Types B and D were previously thought to be exclusive to livestock, where they can produce a disease that looks very similar to MS.