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Posted: Thu Nov 06, 2008 6:18 am Post subject: quercetin inhibits myeloperoxidase
as does rutin but not as much as the plant pterogyne nitens.
Quote:
: Phytochemistry. 2008 May;69(8):1739-44. Epub 2008 Apr 7. Links
Flavonols from Pterogyne nitens and their evaluation as myeloperoxidase inhibitors.Regasini LO, Vellosa JC, Silva DH, Furlan M, de Oliveira OM, Khalil NM, Brunetti IL, Young MC, Barreiro EJ, Bolzani VS.
Universidade Estadual Paulista, Instituto de Química, NuBBE - Núcleo de Bioensaios, Biossíntese e Ecofisiologia de Produtos Naturais, Araraquara, SP, CP 355, CEP 14801-970, Brazil.
A myeloperoxidase inhibitory kaempferol derivative, namely pterogynoside (1), was isolated from fruits of Pterogyne nitens, along with six known flavonols, kaempferol, afzelin, kaempferitrin, quercetin, isoquercetrin and rutin. The structures of all compounds were elucidated primarily from 1D and 2D NMR spectroscopic analyses, as well as by high resolution mass spectrometry. All flavonols were screened to identify secondary metabolites as potential myeloperoxidase (MPO) inhibitors, and at concentrations of 0.50-50nM, quercetin (5), isoquercitrin (6) and rutin (7) exhibited strong inhibitory effects with IC50 values of 1.22+/-0.01, 3.75+/-0.02 and 3.60+/-0.02, respectively. The MPO activity detected for the new derivative 1 was markedly decreased (IC(50) 10.3+/-0.03) when compared with known flavonols 5-7, and interestingly increased when tested against ABTS scavenging activity.
1: Neurosci Lett. 2008 Oct 24;444(2):195-8. Epub 2008 Aug 15. Links
Elevated myeloperoxidase activity in white matter in multiple sclerosis.Gray E, Thomas TL, Betmouni S, Scolding N, Love S.
MS Laboratories, Burden Centre, University of Bristol Institute of Clinical Neurosciences, Frenchay Hospital, Bristol BS16 1JB, United Kingdom.
Recent studies have revealed extensive axonal damage in patients with progressive multiple sclerosis (MS). Axonal damage can be caused by a plethora of factors including the release of proteolytic enzymes and cytotoxic oxidants by activated immune cells and glia within the lesion. Macrophages and microglia are known to express myeloperoxidase (MPO) and generate reactive oxygen species during myelin phagocytosis in the white matter. In the present study we have measured MPO levels in post-mortem homogenates of demyelinated and non-demyelinated regions of white matter from nine patients with MS and seven controls, and assessed MPO immunoreactivity within MS brain. In homogenates of MS white matter, demyelination was associated with significantly elevated MPO activity when compared to controls. Immunohistochemistry showed MPO to be expressed mainly by macrophages within and adjacent to plaques. Demyelination in MS is associated with increased activity of MPO, suggesting that this production of reactive oxygen species may contribute to axonal injury within plaques.
PMID: 18723077 [PubMed - in process]
link _________________ 1st traceable symptoms Jan 01, last edss by doctor 6.5. Feeling better on ginkgo, salvia, capsaicin, curcumin, scutellaria. Interested in other vascular strengthening herbs; pycnogenol, butcher's broom, horsechestnut, centenella, hersperidin
Joined: Sep 11, 2007 Posts: 677 Location: southern California
Posted: Thu Nov 13, 2008 6:40 pm Post subject:
Myeloperoxidase is a green heme protein, and is what makes your phlegm or pus green when you have an infection....yuck!
Gotta say, Jeff's daily EGCG/quercetin combo is making him feel really terrific. I know Lance Armstrong is hawking this combo in his FRS energy drink, but it's possible to buy them as supplements. _________________ Husband diagnosed RRMS March 2007
pursuing endothelial healing
Copaxone, Swank, supplements, laughter
Cheer as you know quercetin with EGCG reduces uric acid levels and helps patients with gout so wonder how it can be so helpful in MS while works contrary to inosine?
I ordered though both and plan to give them to my wife every morning different hours from inosine for the above reason.
Joined: Sep 11, 2007 Posts: 677 Location: southern California
Posted: Fri Nov 14, 2008 12:01 pm Post subject:
DIM wrote:
Cheer as you know quercetin with EGCG reduces uric acid levels and helps patients with gout so wonder how it can be so helpful in MS while works contrary to inosine?.
I didn't know this, Dmitris. You're right, EGCG has been shown to block xanthine oxidase (XO) the precursor to Uric Acid.
Here's some more info on EGCG from the doctor in Berlin who is seeing good results with MS patients-
"As its structure implicates additional antioxidative properties, EGCG is capable of directly protecting against neuronal injury in living brain tissue induced by N-methyl-D-aspartate (NMDA), and of directly blocking the formation of neurotoxic reactive oxygen species in neurons."
Dr. Orhan Aktas
My observations are purely anecdotal. Jeff had been taking inosine for 18 months prior to starting EGCG and quercetin. He was still suffering debilitating fatigue. Since begin EGCG and quercetin in August, he has improved 100%, to the point of no longer needing provigil to stay awake. He visited his parents last week, and his mother called to tell me it was wonderful to see her son full of energy and life again. This combination of antioxidants has worked for him...but for the life of me, I don't know why. Time to get that molecular biology degree
I hope it helps your dear wife!
AC _________________ Husband diagnosed RRMS March 2007
pursuing endothelial healing
Copaxone, Swank, supplements, laughter
Ok Cheer you convince me to stop wifes inosine when EGCG-Quercetin is here although I give her daily pycnogenol (100mg), resveratrol(50mg) and gingko (50mg), all help endohtelium function.
As my wife has some of Jeff's symptoms, say brown spots and increased liver enzymes hope your combination will be helpful for us, by the way the EGCG I ordered is 350mg/caps from Source Naturals so it contains large amounts of caffein, am I wrong?
Joined: Sep 11, 2007 Posts: 677 Location: southern California
Posted: Sat Nov 15, 2008 1:11 pm Post subject:
DIM wrote:
by the way the EGCG I ordered is 350mg/caps from Source Naturals so it contains large amounts of caffein, am I wrong?
Source Naturals has 16g of caffeine. You can get Teavigo, which is caffeine free and has 50mg. EGCG per tablet.
Jeff has responded the best to NSI GreenSelect 600mg. (100mg. EGCG) - which is also caffeine free. It is bioavailable with added "phytosome." He takes one tablet with a tablet of quercetin (500mg. by Jarrow) in 2 hour intervals...(11am, 1pm, 3pm) for a daily total of 1500mg. quercetin and 300mg. EGCG.
I am not sure what is available to you in Greece... _________________ Husband diagnosed RRMS March 2007
pursuing endothelial healing
Copaxone, Swank, supplements, laughter
Unfortunately (for us) Cheer I order all my supplements from USA cause they cost me fewer money although I pay shippings and taxes!
I read that in clinical trials they use 700mg EGCG with 500mg quercetin and plan to give the same amount to my wife although Source Naturals contains as you say large amounts caffein, in worst case I'll give her 350mg EGCG with 500mg quercetin.
I have read very bad comments about NSI products and their purity so have a look (for example their curcumin has many toxic ingredients).
Joined: Sep 11, 2007 Posts: 677 Location: southern California
Posted: Sun Nov 16, 2008 9:32 am Post subject: Re: EGCG & Caffeine
NHE wrote:
cheerleader wrote:
Source Naturals has 16g of caffeine. You can get Teavigo, which is caffeine free and has 50mg. EGCG per tablet.
I hope that you really meant to write 16mg and not 16g (the latter dosage might very well be lethal).
NHE
bingo. MG!
g. wold be quite an energy enhancer! _________________ Husband diagnosed RRMS March 2007
pursuing endothelial healing
Copaxone, Swank, supplements, laughter
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