Barts blog: EBV and B-cells again
Posted: Tue Aug 21, 2018 3:53 am
B cell Cytokines in MS brain, may support B cell follicles.
http://multiple-sclerosis-research.blog ... n-may.html
Excerpts:
Although B cell depletion is an effective therapy of multiple sclerosis (MS), the pathogenic functions of B cells in MS remain incompletely understood. We asked whether cerebrospinal fluid (CSF) B cells in MS secrete different cytokines than control-subject B cells and whether cytokine secretion affects MS phenotype.
We blindly studied CSF B cells after their immortalization by Epstein-Barr Virus (EBV) in prospectively-collected MS patients and control subjects with other inflammatory-(OIND) or non-inflammatory neurological diseases (NIND) and healthy volunteers (HV). The pilot cohort (n = 80) was analyzed using intracellular cytokine staining (n = 101 B cell lines [BCL] derived from 35 out of 80 subjects).
[...]
In this study they found that B cells from people with MS were much more susceptible to infection with EBV and transformation of the B cell. Although the reasons for this was not investigated, it could be that the genetics of people with MS make them more susceptible to infection, or perhaps more susceptible to be activated once infected. There are differences in infection with different HLA-DR variants in some studies and this would be consistent with a hypothesis that EBV may confer better immune responsiveness to infection, which would support co-evolution theory of virus and humans by ProfG
In this study the majority of CSF B cells (87.2%) were memory B cells, including CD27-/IgD- memory tissue-based B cells, expressing CD80 and CD86 cell activation markers TNF-α and LT-α were strongly co-expressed by the same B cells.
And related to this
Secretory products of multiple sclerosis B cells are cytotoxic to oligodendroglia in vitro
https://www.sciencedirect.com/science/a ... 281200063X
http://multiple-sclerosis-research.blog ... n-may.html
Excerpts:
Although B cell depletion is an effective therapy of multiple sclerosis (MS), the pathogenic functions of B cells in MS remain incompletely understood. We asked whether cerebrospinal fluid (CSF) B cells in MS secrete different cytokines than control-subject B cells and whether cytokine secretion affects MS phenotype.
We blindly studied CSF B cells after their immortalization by Epstein-Barr Virus (EBV) in prospectively-collected MS patients and control subjects with other inflammatory-(OIND) or non-inflammatory neurological diseases (NIND) and healthy volunteers (HV). The pilot cohort (n = 80) was analyzed using intracellular cytokine staining (n = 101 B cell lines [BCL] derived from 35 out of 80 subjects).
[...]
In this study they found that B cells from people with MS were much more susceptible to infection with EBV and transformation of the B cell. Although the reasons for this was not investigated, it could be that the genetics of people with MS make them more susceptible to infection, or perhaps more susceptible to be activated once infected. There are differences in infection with different HLA-DR variants in some studies and this would be consistent with a hypothesis that EBV may confer better immune responsiveness to infection, which would support co-evolution theory of virus and humans by ProfG
In this study the majority of CSF B cells (87.2%) were memory B cells, including CD27-/IgD- memory tissue-based B cells, expressing CD80 and CD86 cell activation markers TNF-α and LT-α were strongly co-expressed by the same B cells.
And related to this
Secretory products of multiple sclerosis B cells are cytotoxic to oligodendroglia in vitro
https://www.sciencedirect.com/science/a ... 281200063X