Biomarker: N-glucosylation in Multiple Sclerosis
Biomarker: N-glucosylation in Multiple Sclerosis
https://www.mdpi.com/2076-3425/10/7/453
Abstract
Diagnostics of Multiple Sclerosis (MS) are essentially based on the gold standard magnetic resonance imaging. Few alternative simple assays are available to follow up disease activity. Considering that the disease can remain elusive for years, identification of antibodies fluctuating in biological fluids as relevant biomarkers of immune response is a challenge.
In previous studies, we reported that anti-N-glucosylated (N-Glc) peptide antibodies that can be easily detected in Solid-Phase Enzyme-Linked ImmunoSorbent Assays (SP-ELISA) on MS patients’ sera preferentially recognize hyperglucosylated adhesin of non-typeable Haemophilus Influenzae. Since multivalency can be useful for diagnostic purposes to allow an efficient coating in ELISA, we report herein the development of a collection of Multiple N-glucosylated Peptide Epitopes (N-Glc MEPs) to detect anti-N-Glc antibodies in MS. To this aim, a series of N-Glc peptide antigens to be represented in the N-GlcMEPs were tested in competitive ELISA. We confirmed that the epitope recognized by antibodies shall contain at least 5-mer sequences including the fundamental N-Glc moiety.
Using a 4-branched dendrimeric lysine scaffold, we selected the N-Glc MEP 24, carrying the minimal epitope Asn(Glc) anchored to a polyethylene glycol-based spacer (PEG) containing a 19-atoms chain, as an efficient multivalent probe to reveal specific and high affinity anti-N-Glc antibodies in MS.
Conclusions
The present study reported the development of new N-glucosylated Multivalent Epitope Peptides for the detection of anti-N(Glc) antibodies in Multiple Sclerosis. After a preliminary screening of several short linear peptide epitopes and multiple epitope peptides, the promising results suggest that multivalent interaction can be useful in designing SP-ELISA to detect antibodies to aberrant N-glucosylation in Multiple Sclerosis for both diagnostic and prognostic purposes. Indeed, the multivalent N-Glc MEP 24 ligand, carrying the minimal glucosylated epitope Asn (Glc) anchored to the 19-atoms PEG-based spacer, can be an efficient probe to reveal both IgM and IgG autoantibodies as disease biomarkers.
-
- Similar Topics
- Replies
- Views
- Last post
-
- 1 Replies
- 930 Views
-
Last post by Petr75
Sat Nov 02, 2019 9:51 am
-
- 0 Replies
- 328 Views
-
Last post by Petr75
Sun Oct 18, 2020 6:20 am
-
-
A unified view of multiple sclerosis
by frodo » Sat Jan 30, 2021 3:48 am » in MS Etiology and Pathogenesis - 0 Replies
- 546 Views
-
Last post by frodo
Sat Jan 30, 2021 3:48 am
-
-
- 0 Replies
- 465 Views
-
Last post by Petr75
Thu Mar 19, 2020 10:24 am
-
-
Multiple Sclerosis and Ischemic Stroke
by Petr75 » Fri Mar 29, 2019 10:23 am » in General Discussion - 0 Replies
- 823 Views
-
Last post by Petr75
Fri Mar 29, 2019 10:23 am
-
-
-
Exercise and the brain in multiple sclerosis
by NHE » Sat Nov 14, 2020 4:31 am » in Exercise and Physical Therapy - 0 Replies
- 309 Views
-
Last post by NHE
Sat Nov 14, 2020 4:31 am
-
-
- 0 Replies
- 408 Views
-
Last post by Petr75
Thu Dec 10, 2020 11:35 am
-
-
Type 1 diabetes (T1D) and multiple sclerosis
by Petr75 » Thu Jan 09, 2020 4:23 am » in General Discussion - 1 Replies
- 674 Views
-
Last post by frodo
Wed Jan 15, 2020 4:02 am
-
-
-
Recent advances in understanding multiple sclerosis
by frodo » Tue Dec 17, 2019 1:16 am » in MS Etiology and Pathogenesis - 0 Replies
- 474 Views
-
Last post by frodo
Tue Dec 17, 2019 1:16 am
-