Novel blood-based biomarker for MS
Novel blood-based biomarker for MS
https://journals.sagepub.com/doi/full/1 ... 7320946784
Background
In multiple sclerosis (MS), clinical assessment, MRI and cerebrospinal fluid are important in the diagnostic process. However, no blood biomarker has been confirmed as a useful tool in the diagnostic work-up.
Objectives
Blood platelets contain a rich spliced mRNA repertoire that can alter during megakaryocyte development but also during platelet formation and platelet circulation. In this proof of concept study, we evaluate the diagnostic potential of spliced blood platelet RNA for the detection of MS.
Methods
We isolated and sequenced platelet RNA of blood samples obtained from 57 MS patients and 66 age- and gender-matched healthy controls (HCs). 60% was used to develop a particle swarm-optimized (PSO) support vector machine classification algorithm. The remaining 40% served as an independent validation series.
Results
In total, 1249 RNAs with differential spliced junction expression levels were identified between platelets of MS patients as compared to HCs, including EPSTI1, IFI6, and RPS6KA3, in line with reported inflammatory signatures in the blood of MS patients. The RNAs were subsequently used as input for a MS classifier, capable of detecting MS with 80% accuracy in the independent validation series.
Conclusions
Spliced platelet RNA may enable the blood-based diagnosis of MS, warranting large-scale validation.
-
- Similar Topics
- Replies
- Views
- Last post
-
-
prognosis based in a blood test
by frodo » Tue Jun 16, 2020 2:33 am » in MS Etiology and Pathogenesis - 0 Replies
- 348 Views
-
Last post by frodo
Tue Jun 16, 2020 2:33 am
-
-
- 0 Replies
- 336 Views
-
Last post by Petr75
Fri Oct 09, 2020 8:59 am
-
- 0 Replies
- 243 Views
-
Last post by frodo
Wed May 13, 2020 8:06 am
-
- 0 Replies
- 272 Views
-
Last post by frodo
Mon May 11, 2020 3:24 am
-
- 0 Replies
- 202 Views
-
Last post by frodo
Tue Nov 10, 2020 5:13 am
-
- 2 Replies
- 608 Views
-
Last post by frodo
Wed Jul 31, 2019 11:08 pm
-
- 0 Replies
- 204 Views
-
Last post by frodo
Tue Nov 03, 2020 6:04 am
-
-
Neurofilament light as biomarker
by frodo » Tue May 28, 2019 6:26 am » in MS Etiology and Pathogenesis - 0 Replies
- 525 Views
-
Last post by frodo
Tue May 28, 2019 6:26 am
-
-
-
biomarker for response to IFNbeta
by frodo » Wed Aug 12, 2020 10:58 am » in MS Etiology and Pathogenesis - 0 Replies
- 264 Views
-
Last post by frodo
Wed Aug 12, 2020 10:58 am
-