This pathogen, Clostridium perfringens, has been associated with a lack of protease in animals. For instance, in the following study low trypsin (trypsin is a protease) was listed as a predisposing factor in the development of Clostridium perfringens infections in animals.
J Vet Med B Infect Dis Vet Public Health. 2004 Dec;51(10):423-6.
Significance of beta 2-toxigenic clostridium perfringens infections in animals and their predisposing factors
Schotte U, Truyen U, Neubauer H.
"The novel beta 2-toxin of Clostridium perfringens has recently been described as the cause of enteric diseases in animals. The biological activity of beta 2-toxin is similar to that of the beta1-toxin with a possibly weaker cytotoxic activity. However, the production of beta 2-toxin in vitro is not seen in all beta 2-toxin-gene (cpb2)-positive C. perfringens strains, and to deduce a clinical importance solely from the detection of cpb2 is difficult. Detection of cpb2-positive C. perfringens from various animal species with and without enteric diseases demonstrates the wide distribution of cpb2 in nature, and the presence of cpb2 gene is therefore not considered a risk by itself. Predisposing factors like low trypsin activity in the intestinal tract, antibiotic and/or antiphlogistic treatment or changes in diet can result in the selection of beta 2-toxigenic C. perfringens which may lead to enteritis or enterotoxaemia.
The following information from the Merck Manual states: "This toxin is sensitive to proteolytic enzymes, and disease is associated with an inhibition of proteolysis in the intestine". In addition, it states that colostrum, which contains a trypsin inhibitor has been suggested as a factor for increasing the disease susceptibility of young piglets. Enterotoxemia Caused by Clostridium perfringens Types B and C
"Infection with Clostridium perfringens types B and C causes severe enteritis, dysentery, toxemia, and high mortality in young lambs, calves, pigs, and foals (see Clostridial Diseases: Enterotoxemia Caused by Clostridium perfringens Types B and C). Types B and C both produce the highly necrotizing and lethal beta toxin that is responsible for severe intestinal damage. This toxin is sensitive to proteolytic enzymes, and disease is associated with inhibition of proteolysis in the intestine. Sow colostrum, which contains a trypsin inhibitor, has been suggested as a factor increasing the susceptibility of young piglets.
Type C also causes enterotoxemia in adult cattle, sheep, and goats."http://www.merckmanuals.com/vet/general ... emias.html
The following information from Wiki states that trypsin shortages in the digestive system of experimental animals has been used to "induce" Clostridium perfringens type C.
"Clostridium perfringens beta toxin one of the four major lethal toxins produced by Clostridium perfringens Type B and Type C strains...C. perfringens beta toxin is susceptible to breakdown by proteolytic enzymes, particularly trypsin. Beta toxin is therefore highly lethal to infant mammals because of trypsin inhibitors present in the colostrum...It is primarily fatal to animals 1–3 days old, whose digestive enzymes may not be sufficiently active to break down beta toxin. It has been experimentally shown that trypsin may normally break down beta toxin and trypsin shortages in the digestive system of experimental animals has been used to induce type C disease."
In the following information it states that ingestion of a protein-rich diet in a protease-deficient
intestinal tract allows for "rapid growth" of Clostridium perfringens organisms.
Managing Clostridial Diseases in Cattle
Sheila M. McGuirk, DVM, PhD
"Possible risk factors for calves
o Ingestion of C. perfringens in the first few days of colostrum feeding
o Ingestion of protein-rich diet in a protease-deficient intestinal tract allows rapid growth of C. perfringens organisms"http://www.vetmed.wisc.edu/dms/fapm/fap ... ridial.pdf
The following information states that the digestive enzyme trypsin is a "Clostridium-destroying" digestive enzyme.
Perfringens Type C usually occurs in newborns, as they have a low population of protective normal gut flora and their diet is colostrum, which contains an inhibitor of the Clostridium-destroying digestive enzyme trypsin.http://vads.vetmed.vt.edu/demos/Educati ... PerfFS.htm
I have been making some posts under the thread "Some Interesting Connections" on how protease and another digestive enzyme called DNase 1 is involved in the MS disease process.