Cord Blood

Discuss stem cells, adult and embryonic, and their therapeutic potential for MS here.

Cord Blood

Postby Punchy » Sun May 25, 2008 2:58 pm

If you had children fairly recently, did you choose to save the cord blood?

Why or why not? Would you with future children?

I confess I know very little about stem cells and MS, but I am intrigued by the possibility of doing this with my future children.
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Postby mrhodes40 » Sun May 25, 2008 4:27 pm

We debated saving my grandson's in case someone needed it. I would not have wanted to use it for me: what if my darlings needed it themselves?

It is expensive also. APparently about 2k to start and a monthly rent for space in the cryo.

That having been said, wouldn't it be cool if you could just say"DO a total reboot of me; I'll get my old cord blood and have fresh perfect immunity"
That'd be a cool opportunity.
marie
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Postby StemHope » Thu May 29, 2008 10:48 am

Stem Cell Transplantation

Cord Blood Stem Cells: CBSC have the remarkable potential to develop into many different cell types. Working as Bone Marrow Stem Cells, a sort of repair system for the body, they can theoretically divide without limit to replenish other cells, at the sole condition that the receiving person is alive. When a stem cell divides, each new cell has the potential to either remain a stem cell or become another type of cell with a more specialized function, such as a muscle cell, a red blood cell, or a brain cell.


Umbilical Cord Blood Preparation

The umbilical cord blood from healthy full term newborns with healthy mothers is the only source of stem cells. Given the woman’s consents to donate her umbilical cord, immediately after the delivery, authorized hospital personnel insert a needle into the cord’s vein and the blood is drawn, normally from 80 to 120 ml. The cord blood is then put into a small, sterile pouch with an anticoagulant to prevent clotting. The pouch contains from 100,000 to 300,000 stem cells.

The umbilical cord blood is then immediately sent to the blood bank for testing. First of all, a medical history of the mother is reviewed. Then, the blood is tested to ensure that no genetic disorders, microbiological contamination and/or hereditary diseases exist, such as HIV, Syphilis, CMV, HTLV and Hepatitis. Provided the blood passes all tests, it receives a certification of purity and is then transferred to laboratory.

Here, the blood is tested one more time to confirm the blood banks results. This double check ensures total safety to our patients. After the blood has been cleared it undergoes a process known as magnetic washing, aimed at removing all material except the specifically desired stem cells by means of magnetic beads coated with antibodies to stem cell antigens.



Magnetic enrichment can process very large samples (billions of cells) in one run; significant levels of contaminants (such as T-cells or tumor cells) remain present. FACS results in very pure cell populations that can be selected for several parameters simultaneously (e.g., Linneg, CD34pos, CD90 pos), but it is more time consuming (10,000 to 50,000 cells can be sorted per second) and requires expensive instrumentation.


Purified Cord Blood Stem Cell
When this immunomagnetic separation is done, stem cells are then cultured in a solution containing nutrients and growth factors, but no animal products or by-products: this medium is changed every 1 or 2 days. The cells proliferate for 7 to 10 days under continuous control: if there is any sign of unwanted behavior or contamination, the entire batch is immediately destroyed.
The cells are harvested at the optimum point, i.e. before they start differentiating into specialized cells. At this phase, they normally count from 8 to 10 million. The cells pass through another washing process to remove all growth medium and particles, and we have finally obtained extremely pure stem cells ready for transfusion. If they are not to be used immediately, they are frozen in liquid Nitrogen. Stem cells from umbilical cord blood have the advantage of a “naïve” immune status: in other words, they won’t attack the recipient person. In the rare case rejections were to occur, there are treatments to handle it.

It is important to note that GVHD (Graft Versus Host Disease: a potentially fatal rejection case) has never been an issue with the type of cells (umbilical) we are discussing.


Stem Cells for Ataxia, Stroke, Cerebral Palsy, Multiple Sclerosis and Spinal Cord Injury
Where do stem cells come from?
One source of stem cells is from umbilical cord blood. Since 1988, cord blood stem cells (CBSC) have been used for the treatment of blood disorders and blood cancers, and become available at particular hospitals and universities.
Purified and Potentiated CBSC
Today, modern scientific practices have allowed for the full removal of the white blood cells which can cause GVHD. Consequently HLA matching is no longer required and treatment using this technique carries no associated risks.
Benefits of Purified CBSC
- Create a standardization of data required for efficient clinical trial assessment
- No need for HLA matching or risk of GVHD
- Ease of laboratory expansion (quick dividers) in order to create the high numbers of cells required for efficacious therapeutic application.
The purified stem cells available at selected clinics have been developed using certified third party contract laboratories which detail: phenotype (cell type), purity, total cell count, viability and safety (via genetic level PCR testing for infectious diseases).

Fresh Cell Treatment:
Vain injections under ambient temperature 4 times, every week. The fresh cells must be used immediately with in 24 hours since relocated from the Lab.

Stem Cells for Anti Aging
Potentiated of Cord Blood Stem Cells: CBSC
Used internationally by Piyavate - Shanghai-Heidelberg International, CBSC have been shown to provide significant clinical benefits in the treatment of a wide array of neurological disorders, including spinal cord injuries and strokes, diabetes, as well as heart, lung, autoimmune, skin, ocular and viral conditions.
when jection of fresh and purified CBSC into the body of an aged person will initiate repair and regeneration of aged organs and cells in this person.



Emerging Clinical Fresh Cells Applications
Stroke:

Doctors are focusing on the ability of the stem cell to differentiate into nerve cells. The approach to treatment would be to regenerate new and healthy nerve cells (tissue) to reduce, alleviate, or eliminate the symptoms or effects of diseases like Alzheimer’s and Parkinson’s and repair damage caused by stroke.

Sanchez-Ramos, J., Song, S., Kamath, S., et al. Expression of neural markers in human umbilical cord blood. Experimental Neurology. 2001;171:109-115.
Chen, J., Sanberg, P., Li, Y., et al. Intravenous administration of human umbilical cord blood reduces behavioral deficits after stroke in rats. Stroke. 2001;32:2682-2688.

Spinal Cord Injury:
In animal studies cord blood stem cells are beneficial in helping to reverse paralysis caused by spinal cord injury. Cord blood-derived stem cells were shown to migrate to and participate in the healing of neurological defects caused by traumatic assault.
Saporta, S., Kim, J., Willing, A., et al. Human umbilical cord blood stem cells infusion in spinal cord injury: engraftment and beneficial influence on behavior. Journal of Hematotherapy & Stem Cell Research. 2003; 12:271-278.


Diabetes:
Successful regeneration of pancreatic function and insulin production has been achieved using stem cells. May help insulin dependent diabetic patients become free of adjunctive therapy (such as insulin injections).
Domenick, M., Ildstad, S. Impact of bone marrow transplantation on type I diabetes. World Journal of Surgery. 2001;25:474-480.
Heart Disease:
Successful regeneration of heart tissue and blood vessels for the treatment of heart disease or traumatic injury to the heart.
Assmus, B., Schachinger, V., Teupe, C., et al. Transplantation of progenitor cells and regeneration enhancement in acute myocardial infarction (TOPCARE-AMI). Circulation. 2002;106:3009-3017.
Liver Disease:
Stem cells have been differentiated into liver cells in mice. Future human applications may include repairing liver damage caused by cirrhosis, viral infection, trauma, chemotherapy, or radiation therapy.
Wang X., Ge S., McNamara G., et al. Albumin-expressing hepatocyte-like cells develop in the livers of immune-deficient mice that received transplants of highly purified human hematopoietic stem cells. Blood. 2003;101(10):4201-4208.
Lupus:
Following stem cell transplantation patients with severe lupus have been able to regenerate blood cells that are completely free of the disease.
Brunner, M., Greinix, H., Redlich, K., et al. Autologous blood stem cell transplantation in refractory systemic lupus erythematosus with severe pulmonary impairment. Arthritis & Rheumatism. 2002;46(6):1580-1584.

Multiple Sclerosis:
Stem cell transplantation has stopped and even partially reversed disease progression for some patients with multiple sclerosis.
Kozak, T. Hematopoietic stem cell transplantation in the treatment of autoimmune diseases. The Middle European Journal of Medicine. 2002;114(1-2; 7-13)
Muscular Dystrophy:
Stem cells have been proven to fuse into skeletal muscle cells in patients with muscular dystrophy. Stem cell transplantation may help slow or stop the progression of muscle wasting.
Gussoni, E., Bennett, R., Muskiewicz, K., et al. Long term persistence of donor nuclei in a duchenne muscular dystrophy patient receiving bone marrow transplantation, The Journal of Clinical Investigation, 2002;110(6):807-814.

Applications of Purified CBSC:
listed by the American Association of Blood Banks as follows:
Malignant Diseases
• Acute lymphocytic leukemia
• Acute myelocytic leukemia
• Juvenile chronic myelogenous leukemia
• Chronic myelogenous leukemia
• Neuroblastoma
• Refractory anemia with excess blasts
Nonmalignant Diseases
• Fanconi anemia
• Aplastic anemia
• Refractory anemia
• Thalassemia
• Sickle cell anemia
• Amegakaryocytic thrombocytopenia
• Kostmann syndrome
• Blackfan-Diamond syndrome
• Severe combined immunodeficiency
• X-linked lymphoproliferative disorder
• Wiskott-Aldrich syndrome
• Hurler syndrome
• Hunter syndrome
• Gunther disease
• Osteopetrosis
• Globoid cell leukodystrophy
• Adrenoleukodystrophy
Usage: Intravenous injections under ambient temperature, 1-2 times. The fresh cells must be used within 24 hours of leaving the laboratory.
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Postby Jamie » Thu May 29, 2008 9:02 pm

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Postby StemHope » Fri May 30, 2008 10:08 am

Wow this is the second time I have posted information on this board and have had some smart a$$ remark. I guess its to be expected. But, after overlooking the definition of quackery, I see that it someone that claims to be a pro on the area, which I never claimed. Everything I posted above gives credentials to their source. So I guess we need a site like www.if_you_have_nothing_nice_to_say.com. I love forums there are always the person on these things that has something to prove. Congratulations you really put me in my place, I make a comment directly related to the topic and you offered your insightful 2 cents horray.

So I guess let me ask you then, say you just had a baby. Well, 7 years down the road your kid developes a disease that the only possible option that your kid has to live is through Stem Cell treatment. Let me guess you would be the one that wouldnt use it right?? Yeah You would be too proccupied with how you could take your time to bust my chops about being a quack. Thanks and wish you all the best. You are the person that still says we never made it to the moon or whatever, I feel sorry for you.
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Postby cheerleader » Fri May 30, 2008 4:00 pm

Hey Stem Hope-
Welcome.
Don't take it personally. We're all just a bit wary of new folks posting such lengthy "position papers" right off. Maybe post the link, and let us go to the site. And don't feel sorry for Jamie, he can't help it, he's British :)

Maybe spend some time here, introduce yourself on the introductions thread. Let folks get to know you and your story (in less than 200 words, if possible.) I'm all for stem cell reserch....gave my placenta and son's umbilical cord to a French research team 13 years ago. Hope my kid won't want it back!

all the best,
AC
Husband dx RRMS 3/07
dx dual jugular vein stenosis (CCSVI) 4/09
http://ccsviinms.blogspot.com
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Cord Blood

Postby Brainteaser » Fri May 30, 2008 8:04 pm

Just to say that my son and his partner have recently had a baby. I suggested that the cord blood be saved and they agreed. I'm paying for the blood to be stored and in Oz it costs about $2500 plus $150 per year (A$1=approx US$1), payable on the child's birthday (birthday present).

I think it is a reasonable thing to do, especially as the likelihood of MS for my son and grandson are higher than normal. Over a lifetime the costs are not astronomical - I can't see the cord blood being of benefit to me personally but hopefully it is a useful insurance measure and it gives some comfort to them later on if things turn bad with their health.

Phil
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Postby Punchy » Sat May 31, 2008 8:58 am

Well I must admit, I just finished reading the book "My Sister's Keeper". :)

And I wasn't thinking of using the blood for my own treatment, but as a sort of insurance policy for my future kids.
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Are you sure stem cells cure MS?

Postby superman » Sun Jun 01, 2008 8:22 am

I am not a doctor, but i find it quite a big gamble to keep a blood cord for somebody's life, especially when you have to pay so much.
Is there anything that certify you blood cord cure you.
I have great hope about stem cells.
But keeping blood cord remind me "Gattaca" movie.
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Postby Punchy » Sun Jun 01, 2008 11:04 am

The question is if I don't do it, and my child ends up with leukemia or ppms or some other godawful condition, will I be able to forgive myself after considering it and deciding against it?
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Postby StemHope » Tue Jun 03, 2008 8:55 am

I will post in the intro lounge next, but I decided to follow advice and post links. This site shows a lot of case studies on people with many degenerative disease and how stem cells have helped them.

Also it cost less to use a donors cord blood, its not from you but, it doesnt cost a fortune to store and it is still extremely effective. Im sure these videos will brightnen some days, the story of Denise is most like my situation. My hand and wrist pain would bring me to tears in bed all during the night.

http://www.beike.ch/en/Multiple-Sclerosis/
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Postby mrhodes40 » Tue Jun 03, 2008 9:50 am

Thank yo stemhope for making yourself known to us. I am interested in what you have to share, you've obviously done some different things.
marie
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