Epstein-Barr Virus and miRNAs
Epstein-Barr Virus and miRNAs
Department of Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates
Epstein-Barr Virus and miRNAs: Partners in Crime in the Pathogenesis of Multiple Sclerosis?
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456696/
Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that modulate gene expression post transcriptionally. In healthy individuals, miRNAs contribute to maintaining gene expression homeostasis. However, the level of miRNAs expressed is markedly altered in different diseases, including multiple sclerosis (MS). The impact of such changes is being investigated, and thought to shape the immune system into the inflammatory autoimmune phenotype. Much is yet to be learned about the contribution of miRNAs in the molecular pathology of MS. Epstein-Barr virus (EBV) infection is a major risk factor for the development of MS. EBV encodes more than 40 miRNAs, most of which have been studied in the context of EBV associated cancers. These viral miRNAs regulate genes involved in cell apoptosis, antigen presentation and recognition, as well as B cell transformation. If EBV infection contributes to the pathology of MS, it is plausible that EBV miRNAs may be involved. Unfortunately, there are limited studies addressing how EBV miRNAs are involved in the pathogenesis of MS. This review summarizes what has been reported regarding cellular and viral miRNA profiles in MS and proposes possible interactions between the two in the development of MS.
Re: Epstein-Barr Virus and miRNAs
The herpes virus causes MS, EBV is a herpes strain.
The recognition of the virus at cellular level is done by SNPs.
And likely via SNPs the miRNA is involved in B cell transformation.
The SNPs function within the miRNA and within miRNA targets.
As such, SNPs modulate miRNA or are associated with miRNA regulation.
The polymorphisms are the result of hundreds of millions of years of evolution.
Fish have some of the same polymorphisms as human beings, indicating they date back from the common root from a long time ago.
And a significant part of our cells have miRNA.
The overall picture may be found here: viewtopic.php?f=1&t=15188&start=885#p257234
Re: Epstein-Barr Virus and miRNAs
Centre for Immunology and Allergy Research, Westmead Institute for Medical Research, University of Sydney, Australia
Evidence from genome wide association studies implicates reduced control of Epstein-Barr virus infection in multiple sclerosis susceptibility.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6492329/
Abstract
BACKGROUND:
Genome wide association studies have identified > 200 susceptibility loci accounting for much of the heritability of multiple sclerosis (MS). Epstein-Barr virus (EBV), a memory B cell tropic virus, has been identified as necessary but not sufficient for development of MS. The molecular and immunological basis for this has not been established. Infected B cell proliferation is driven by signalling through the EBV produced cell surface protein LMP1, a homologue of the MS risk gene CD40.
METHODS:
We have investigated transcriptomes of B cells and EBV-infected B cells at Latency III (LCLs) and identified MS risk genes with altered expression on infection and with expression levels associated with the MS risk genotype (LCLeQTLs). The association of LCLeQTL genomic burden with EBV phenotypes in vitro and in vivo was examined. The risk genotype effect on LCL proliferation with CD40 stimulation was assessed.
RESULTS:
These LCLeQTL MS risk SNP:gene pairs (47 identified) were over-represented in genes dysregulated between B and LCLs (p < 1.53 × 10-4), and as target loci of the EBV transcription factor EBNA2 (p < 3.17 × 10-16). Overall genetic burden of LCLeQTLs was associated with some EBV phenotypes but not others. Stimulation of the CD40 pathway by CD40L reduced LCL proliferation (p < 0.001), dependent on CD40 and TRAF3 MS risk genotypes. Both CD40 and TRAF3 risk SNPs are in binding sites for the EBV transcription factor EBNA2, with expression of each correlated with EBNA2 expression dependent on genotype.
CONCLUSIONS:
These data indicate targeting EBV may be of therapeutic benefit in MS.
Re: Epstein-Barr Virus and miRNAs
Centre for Immunology and Allergy Research, Westmead Institute for Medical Research, The University of Sydney, Westmead, Australia
Transcribed B lymphocyte genes and multiple sclerosis risk genes are underrepresented in Epstein-Barr Virus hypomethylated regions.
https://www.ncbi.nlm.nih.gov/pubmed/31619767
Abstract
Epstein-Barr Virus (EBV) infection appears to be necessary for the development of Multiple Sclerosis (MS), although the specific mechanisms are unknown. More than 200 single-nucleotide polymorphisms (SNPs) are known to be associated with the risk of developing MS. About a quarter of these are also highly associated with proximal gene expression in B cells infected with EBV (lymphoblastoid cell lines-LCLs). The DNA of LCLs is hypomethylated compared with both uninfected and activated B cells. Since methylation can affect gene expression, and so cell differentiation and immune evasion, we hypothesised that EBV-driven hypomethylation may affect the interaction between EBV infection and MS. We interrogated an existing dataset comprising three individuals with whole-genome bisulfite sequencing data from EBV transformed B cells and CD40L-activated B cells. DNA methylation surrounding MS risk SNPs associated with gene expression in LCLs (LCLeQTL) was less likely to be hypomethylated than randomly selected chromosomal regions. Differential methylation was independent of genomic features such as promoter regions, but genes preferentially expressed in EBV-infected B cells, including the LCLeQTL genes, were underrepresented in the hypomethylated regions. Our data does not indicate MS genetic risk is affected by EBV hypomethylation.
-
- Similar Topics
- Replies
- Views
- Last post
-
-
Epstein Bar Virus - A Review of the Epstein Barr Virus in Relationship to MS
by Kinora » Fri May 03, 2019 9:08 am » in MS Etiology and Pathogenesis - 0 Replies
- 172 Views
-
Last post by Kinora
Fri May 03, 2019 9:08 am
-
-
- 1 Replies
- 1172 Views
-
Last post by frodo
Sat Jul 28, 2018 9:07 am
-
- 0 Replies
- 574 Views
-
Last post by frodo
Fri Dec 21, 2018 1:32 am
-
-
Vitamin D levels in MS impact Epstein-Barr viral load
by frodo » Thu Aug 23, 2018 7:20 am » in MS Etiology and Pathogenesis - 1 Replies
- 721 Views
-
Last post by jimmylegs
Thu Aug 23, 2018 8:32 am
-
-
-
miRNAs: Identification of MS-specific serum miRNAs
by frodo » Fri Aug 24, 2018 12:00 pm » in MS Etiology and Pathogenesis - 0 Replies
- 620 Views
-
Last post by frodo
Fri Aug 24, 2018 12:00 pm
-
-
-
Can the JC virus be killed before PML
by gramcjk » Sat Aug 17, 2019 11:56 am » in General Discussion - 21 Replies
- 526 Views
-
Last post by ElliotB
Sun Aug 25, 2019 5:59 am
-
-
- 0 Replies
- 246 Views
-
Last post by Petr75
Mon Feb 25, 2019 10:12 am
-
-
Herpes Virus Reactivation in Astronauts
by Petr75 » Sun Mar 24, 2019 3:16 am » in General Discussion - 0 Replies
- 166 Views
-
Last post by Petr75
Sun Mar 24, 2019 3:16 am
-
-
-
MSRV (MS retro-virus) and EBV interaction
by frodo » Mon Mar 04, 2019 5:28 am » in MS Etiology and Pathogenesis - 1 Replies
- 236 Views
-
Last post by NHE
Tue Mar 05, 2019 12:49 am
-