Source: http://www.sciencedirect.com/science/ar ... 1614000515
Identification of a B cell-dependent subpopulation of multiple sclerosis by measurements of brain-reactive B cells in the blood
Highlights
•CNS-reactive B cells can be detected in the blood of CIS/MS patients by ELISPOT.
•CNS-reactive B cells are absent in healthy control subjects.
•CNS-reactive B cells are absent in neurological and autoimmune control subjects.
•CNS-reactive B cells in the blood possibly indicate B cell-dependent MS.
Abstract
B cells are increasingly coming into play in the pathogenesis of multiple sclerosis (MS). Here, we screened peripheral blood mononuclear cells (PBMC) from patients with clinically isolated syndrome (CIS), MS, other non-inflammatory neurological, inflammatory neurological or autoimmune diseases, and healthy donors for their B cell reactivity to CNS antigen using the enzyme-linked immunospot technique (ELISPOT) after 96 h of polyclonal stimulation. Our data show that nine of 15 patients with CIS (60.0%) and 53 of 67 patients with definite MS (79.1%) displayed CNS-reactive B cells, compared to none of the control donors. The presence of CNS-reactive B cells in the blood of the majority of patients with MS or at risk to develop MS along with their absence in control subjects suggests that they might be indicative of a B cell-dependent subpopulation of the disease.
Blood test to clasify patients?
- HarryZ
- Family Elder
- Posts: 2572
- Joined: Tue May 25, 2004 2:00 pm
- Location: London, ON, Canada
- Contact:
Re: Blood test to clasify patients?
Sure sounds like they are getting closer to a blood test to diagnose MS. With about an 80% accuracy for reactive B cells in MS patients that still means they would have to use this test in combination with other tests to come to a definitive diagnosis.